Fig. 6. IFNγ and cytokines promote elongation and migration of MB231 breast cancer cells.
A Microscope images (10X) of control and cytokine-treated MB231 cells for 48 h. B Cell elongation analysis after 48 h cytokine treatment. C A scratch test assay of MB231 cells after 12 h of cytokine treatment. D Cell invasion assay; MB231 cells were seeded in the upper well of a Boyden chamber with serum-free media ± cytokines and the pan-NOS/COX inhibitors LNAME and indomethacin for 48 h. The lower chamber was filled with complete media. Cells were counted against the standard curve. Results are presented as mean ± SD. *p < 0.05, **p < 0.001, ***p < 0.0001.