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. 2023 May 11;14:2415. doi: 10.1038/s41467-023-38051-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Schematic representation of the N-terminal chimeras of axolotl TRPV1. Chimeric channels were generated between rat (black) and axolotl TRPV1 (Blue) on the basis of the location of the ankyrin repeats (A1–A6). Sequence alignment of Ankyrin Repeat 1 of four tailed amphibians and rat TRPV1. b Chimera (ARNT1-5) or rTRPV1 was expressed in Xenopus oocytes and the representative heat-induced currents were standardized by capsaicin-induced currents at 5 μM are indicated. Average currents for heat stimulation at 38 °C standardized by the capsaicin-induced currents of each chimera in oocytes were compared (n = 5). Each data point represents the mean ± SE. Statistically significant differences were determined by one-way ANOVA with Tukey–Kramer multiple comparison method and different letters (a or b) indicate significant differences between the six groups (p < 0.01). Source data are provided as a Source Data file. c Q128R mutation was introduced in ARNT6 chimera (NT6-Q128R). The sequence of the 137–140AA (EGDK) of axTRPV1 was exchanged with the corresponding sequence (QSNC) of rTRPV1 in the ARNT6 chimera [NT6(137–140Rat)]. Chimera mutants [ARNT6, NT6-Q128R, NT6(137–140Rat)] or rTRPV1 was expressed in Xenopus oocytes and the representative heat-induced currents standardized by the capsaicin-induced currents at 5 μM are indicated. Average currents for heat stimulation at 40 °C standardized by capsaicin-induced currents for each mutant in oocytes were compared (n = 5). Each data point represents the mean ± SE. Statistically significant differences were determined by one-way ANOVA with Tukey–Kramer multiple comparison method and different letters (a or b) indicate significant differences between the three groups (p < 0.05). Source data are provided as a Source Data file. d A R151Q, L154K, or E160S mutation was introduced into the ARNT7 chimera (NT7-R151Q, NT7-L154K, NT7-E160S). Chimera mutants (NT7, NT7-R151Q, NT7-L154K, NT7-E160S) or rTRPV1 were expressed in Xenopus oocytes and the representative heat-induced currents standardized by capsaicin-induced currents at 5 μM are indicated. The average currents for heat stimulation at 38 °C standardized by capsaicin-induced currents for each mutant in oocytes were compared (n = 5). Each data point represents the mean ± SE. Statistically significant differences were determined by one-way ANOVA with Tukey–Kramer multiple comparison method and different letters (a or b) indicate significant differences between the four groups (p < 0.001). Source data are provided as a Source Data file.