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. 2023 Apr 28;14:1105427. doi: 10.3389/fphar.2023.1105427

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Copyright © 2023 Liu, Wang, Huang, Rutz, Ciotkowska, Tamalunas, Hu, Trieb, Waidelich, Strittmatter, Stief and Hennenberg.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of NUAK silencing, HTH01-015 and WZ4003 on contraction of WPMY-1 cells. Contraction of WPMY-1 cells over time was assessed by matrix contraction, starting 48 h after transfection with scramble siRNA or isoform-specific NUAK siRNAs (A), or starting 24 h after addition of HTH01-015 (10 µM), WZ 4003 (10 µM) or solvent to controls (B). Contractions are expressed as decreases in plug diameter during indicated periods. Shown are contractions over time (means with SD), representative pictures for each condition, and single values from each sample and each single experiment for each time point in scatter plots, including p values from one-way ANOVA with Tukey’s test in (A) and one-way ANOVA with Dunnett’s test in (B).