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. 2023 Apr 28;14:1105427. doi: 10.3389/fphar.2023.1105427

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Copyright © 2023 Liu, Wang, Huang, Rutz, Ciotkowska, Tamalunas, Hu, Trieb, Waidelich, Strittmatter, Stief and Hennenberg.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of NUAK silencing, HTH01-015 and WZ4003 on apoptosis and cell death of WPMY-1 cells. Apoptosis and cell death were determined by flowcytometry for annexin V and 7-AAD, 48 h after transfection with scramble siRNA or isoform-specific NUAK siRNAs (A), or 24 h after addition of HTH01-015, WZ4003 or solvent to controls (B). Annexin V-positive/7-AAD-negative cells represent apoptotic cells, wheras annexin V-positive/7-AAD-positive cells represent dead cells. Shown are scatter plots including all single values from each independent experiment together with p values from one-way ANOVA with Tukey’s test in (A), and from one-way ANOVA with Dunnett’s test in (B), and representative experiments.