FIGURE 7.

Effects of HTH01-015 and WZ4003 on marker expression, and on MYPT/MLC phosphorylations in human prostate tissues. RT-PCR for Ki-67 (marker for proliferation), calponin (marker for smooth muscle cells) and cytokeratin-19 (marker for glandular epithelial cells) was performed after incubation of human prostate tissues with HTH01-015 WZ4003 (both 10 µM) or solvent for controls for 22 h together with 50 nM DHT (in all samples) (A) or 5 h (B). Western blot analyses for calponin and pan-cytokeratin were performed after incubation of human prostate tissues with HTH01-015, WZ4003 (both 10 µM) or solvent for controls for 22 h (C). Western blot analyses for phosphorylated and non-phosphorylated MLC and MYPT1 were performed after incubation of human prostate tissues with HTH01-015 (500 nM) or solvent for controls for 30 min (D). Shown are scatter plots including all single values from quantification of each independent experiment together with p values from one-way ANOVA with Dunnett’s test in (A), and Student’s t-test in (D), and representative Western blots.