FIGURE 8.

Effects of HTH01-015 on EFS-induced and adrenergic contractions of human prostate tissues. Contractions of human prostate tissues were induced by EFS (A,B), noradrenaline (C,D), phenylephrine (E,F) or methoxamine (G,H) in an organ bath, 30 min after addition of HTH01-015 in concentrations of 500 nM (A,C,E,G) or 10 µM (B,D,F,H) or of an equivalent amount of DMSO (controls), which was used as solvent for HTH01-015. Shown are data from n = 10 independent experiments in (A) and n = 5 independent experiments in each other subpanel, where tissues from n = 10 or n = 5 patients were used for both groups of a subpanel, resulting in paired samples. Shown are means ± SD from all experiments in concentration response curves with p values from two-way ANOVA for whole groups, and all single E_max, EC₅₀ and Ef₅₀ values from all experiments (calculated by curve fitting) together with p values from Student’s t-test in scatter plots.