Figure 3.

mROS enhance HIF-1α stabilization by inhibiting HIF-1α hydroxylation. (A and B) PASMCs were exposed to normoxia and hypoxia with or without MitoQ (0.5 μM) incubation. (A) mRNA levels of PHD2 and PHD3 relative to β-actin determined by RT-qPCR. (B) Immunoblots for PHD2, PHD3, and β-actin. Data represent mean ± SEM; *P < 0.05, ***P < 0.001 vs. Nx; ^#P < 0.05, ^###P < 0.001 vs. Hx; two-way ANOVA and Bonferroni's post-test. (C) Immunoblots for HIF1α-Pro⁵⁶⁴-OH, total HIF-1α, and β-actin in PASMCs exposed to normoxia and hypoxia with or without MitoQ and MG-132 (10 μM) incubation. Data represent mean ± SEM; *P < 0.05, ***P < 0.001. (D) Immunoblots for HIF1α-Pro⁵⁶⁴-OH, total HIF-1α, and β-actin in PASMCs incubated with exogenous H₂O₂ (0, 50, 75, and 100 μM) and MG-132 (10 μM). Data represent mean ± SEM; *P < 0.05, ***P < 0.001.