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. 2023 Apr 28;14:1178113. doi: 10.3389/fimmu.2023.1178113

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Copyright © 2023 Wang, Liu, Chen and Zhang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Striatal dopaminergic neurons were co-cultured with microglia of different polarization statuses. (A) Striatal dopaminergic neurons are cultured. (B) Immunofluorescence staining of TH expression in different groups: co-cultured with LPS-treated microglia, IL-4 treated microglia, control-animal-derived striatal microglia, and TS-rat-derived microglia. (C) Immunofluorescence staining of DAT expression in different groups. (D) Immunofluorescence staining of PITX3 expression in different groups. (E) Western blot analysis of the expression of TH, DAT, and PITX3 in different co-culture groups. *P <0.05, **P <0.01.