TABLE 2.
Inhibition of B. bovis by bovine Mφ in a phagocytosis-permissive system is partially reversible by the addition of l-NMMA
| Expt no. | % Inhibitiona
|
|
|---|---|---|
| Without l-NMMA | With l-NMMA | |
| 1 | 88 ± 1 | 77 ± 1b |
| 2 | 57 ± 2 | 44 ± 5b |
| 3 | 75 ± 3 | 62 ± 4b |
| 4 | 89 ± 1 | 57 ± 1c |
a
Inhibition of B. bovis replication in the absence or presence of 250 μM l-NMMA was determined as described in the text. The data are presented as the means ± 1 SD of quadruplicate determinations for experiments 1 to 3 and duplicate determinations for experiment 4. Experiments were performed on four occasions and used Mφ derived from two cattle, with an individual animal per experiment.
b
P < 0.05 for comparison with inhibition in the absence of l-NMMA, determined by the Student one-tailed t test.
c
Statistical analysis was not performed because the samples were tested in duplicate.