Characteristics of intact N-glycopeptides in RA and OA synovium.A–C, Venn diagram showing identified N-glycopeptides (A), N-glycosites (B), N-glycoproteins, and (C) in RA and OA samples. D, distribution of all identified N-glycopeptides with N-X-T/S/C tripeptide sequons is shown as the means ± SDs (n = 4 cases per OA or RA group). E, pie charts depict the percentage of N-glycoproteins with the identified number of N-glycosites per protein (upper) and the percentage of N-glycosites with the identified number of N-glycans per glycosite in OA and RA samples (below). F, percentage of the six types of glycans in all N-glycosites. G, number of N-glycosites with different glycan types identified in the OA and RA groups. H, UpSet plot represents the frequency of glycan pairs existing at the same N-glycosite. OA, osteoarthritis; OPLS-DA, orthogonal partial least squares discriminant analysis; RA, rheumatoid arthritis.