Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 May 9;222(8):e202303017. doi: 10.1083/jcb.202303017

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 Meng et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Figure 3. — Wounding triggers the accumulation of the Golgi membrane at the wound site. (A) Diagram showing the location of Golgi apparatus compartment markers and the target of pharmacological inhibitors. (B) Representative confocal images showing the distribution of Golgi apparatus markers before and after wounding. The markers include GFP::RAB-1 (ERGIC), GFP::MANS-2 (medial), GFP::RAB-6.2 (trans), and TGN-38::tagBFP (TGN), respectively. Pcol-19-GFP::RAB-6.2(zjuSi313), Pcol-19-TGN-38::tagBFP(zjuSi280), Pcol-19-GFP::MANS-2(zjuSi302), and Pcol-19-GFP::RAB-1(zjuSi253) transgenic animals were used for wounding and imaging. “W” indicates the wound site. Scale bar: 10 µm. (C) HiS-SiM single-plane images showing the accumulation of TGN-38::tagBFP and GFP::MANS-2 at the wound site. Pcol-19-TGN-38::tagBFP(zjuSi280) and Pcol-19-GFP::MANS-2(zjuSi302) transgenic animals were used for wounding and imaging. Scale bar: 10 µm. (D) Representative single-plane confocal images showing the colocalization of TGN-38::tagBFP and myr::mKate2 before and after wounding. Pcol-19-TGN-38::tagBFP(zjuSi280); Pcol-19-myr::mKate2(zjuSi46) transgenic animals were used for wounding and imaging. “W” indicates the wound site. Scale bar: 10 µm. See also Fig. S3 and Video 9.