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. 2023 Mar 13;193(1):31–47. doi: 10.1093/toxsci/kfad027

Figure 3.

Figure 3.

Adolescent THC exposure increases activation of primordial ovarian follicles. One-month-old female mice were exposed to THC (5 mg/kg, IP) or vehicle (5% Tween 80 in 0.9% saline) for 4 days (PND 30–33) or 14 days (PND 30–43) and ovaries were harvested at PND 34 (24 h after the last injection) or PND 70 (4 weeks after the last injection), respectively. PND 34 and PND 70 ovaries were processed for immunostaining for the proliferation protein Ki67 as detailed in Materials and Methods. Representative images show (A) Ki67-negative follicles in vehicle control ovary (white arrowhead) or (B) Ki67-positive follicles with brown stained granulosa cell nuclei in THC ovary (red arrowheads). The graphs show the means ± SEM percentages of Ki67-positive primordial and primary follicles in PND 34 (C) and PND 70 (D) ovaries; dots show values for individual mice. Administration of THC significantly increased the percentage of primary follicles with Ki67-positive granulosa cells. *p < .05, t test THC versus vehicle. N = 6–8/group. Scale bars, 50 µm.