Figure 4.

Adolescent THC exposure increases DNA damage in ovarian follicles: Female mice were exposed to THC or vehicle as described for Figure 2 and ovaries were processed for immunostaining for γH2AX as described in Materials and Methods. A and B, Representative images show γH2AX-positive primordial and primary follicles with brown stained nuclei in oocytes in THC (red arrowhead) or vehicle-exposed ovary. White arrowheads indicate γH2AX-negative oocytes in vehicle control ovary. The graphs show the means ± SEM percentages of follicles with γH2AX-positive oocytes in PND 34 (C) and PND 70 (D) ovaries; dots show values for individual mice. THC exposure significantly increased the percentages of primary follicles with γH2AX-positive oocytes in PND 34 mice. *p < .05, t test THC versus vehicle. N = 6–8/group. Scale bars, 50 µm.