Skip to main content
. 2023 Mar 13;193(1):31–47. doi: 10.1093/toxsci/kfad027

Figure 5.

Figure 5.

Adolescent THC exposure increases pro-apoptotic PUMA expression in ovarian follicles. Female mice were exposed to THC or vehicle as described for Figure 2, and PND 34 and 70 ovaries were processed immunostaining for p53-upregulated modulator of apoptosis (PUMA) as detailed in Materials and Methods. Representative images of vehicle- (A) and THC-exposed (B) ovaries show PUMA-positive primordial (red arrowhead) and primary (black arrowhead) follicles with brown stained oocytes, as well as a PUMA-negative primary follicle (white arrowhead). C, The graphs show the means ± SEM percentage of PUMA positive primordial and primary follicles in PND34 ovaries. D, The graphs show the means ± SEM percentage of PUMA positive primordial and primary follicles in PND70 ovaries. Dots show values for individual mice. THC exposure significantly increased the percentage of primordial and primary follicles with PUMA-positive oocytes in PND 34 THC mice. *p < .05, t test THC versus vehicle. N = 6–8/group. Scale bars, 50 µm.