Table 1.
Expression of the string-lacZ fusion constructs shown in Fig. 1
| Reporter gene | Cycles 1–13 | Cycle 14 | Cycle 15 | Cycle 16 & later cycles | Larval neuroblast cycles |
|---|---|---|---|---|---|
| pstgHZ | – |
head
stripes along A/P axis, anterior and posterior midgut |
lateral epidermis (11) (partial, weak) | lateral epidermis (11) CNS |
not analyzed |
| pstgβ | – |
head
stripes along A/P axis, anterior and posterior midgut mesoderm |
lateral epidermis (11) (partial, weak) | lateral epidermis (11) CNS |
L2: MBNbs, CB L3: VNCTh,T, CB, aOPC, pOPC |
| pstgβ-B3.2 | – | – | – | – | – |
| pstgβ-2.3 | – | – | – | – | – |
| pstgβ-E2.2 | – | – | lateral epidermis (11) (partial, weak) | lateral epidermis (11) CNS | not analyzed |
| pstgHZ-B8.2 | maternal | head (1, 2) mesoderm (10) mesectoderm (14) neurectoderm (21, N) ventral epidermis (M) |
head (1*, 2*) mesoderm (10) neurectoderm (21, N*) ventral epidermis (M*) CNS |
head mesoderm (10) tracheal placodes neurectoderm (N*) CNS |
not analyzed |
| pstgβ-E4.9 | maternal | mesoderm (10) mesectoderm (14) neurectoderm (21, N) ventral epidermis (M) |
head mesoderm (10) mesectoderm (14) neurectoderm (21, N*) ventral epidermis (M*) |
head mesoderm (10) neurectoderm (N*) PNS |
L2: MBNbs, VNCTh,A,T, CB, OPC, IPC L3: VNCTh,T, CB, aOPC, pOPC |
| pstgHZ-BX7.7 | – | head (1, 2) CNS |
head (1*, 2*) CNS |
head tracheal placodes CNS, PNS |
not analyzed |
| pstgβ-E6.4 | – | head (1, 2, 15, 18) mesectoderm (14) CNS |
head (1*, 2*, 15) posterior tip of germ band (4) CNS |
head tracheal placodes CNS |
L2: MBNbs, VNCTh, CB, OPC, IPC L3: VNCTh,T, CB, interior optic lobe cells |
| pstgHZ-SpEa2.3 | – | head (1, 2) | head (1*, 2*) scattered cells in ventral epidermis |
head tracheal placodes |
not analyzed |
| pstgHZ-BEa1.2 | – | head (1, 2, 15) mesectoderm (14) |
head (1*, 2*, 15) scattered cells in ventral epidermis |
head tracheal placodes |
not analyzed |
| pstgβ-B5.8 | – | CNS | CNS | CNS, PNS | L2: MBNbs, VNCTh, CB L3: VNCTh,T, CB, pOPC, IPC |
| pstgβ-E2.6 | – | CNS | CNS | CNS, PNS | L2: MBNbs, VNCTh,T, CB L3: VNCTh,T, CB, pOPC, IPC |
| pstgβ-E5.3 | – | CNS hindgut (13) |
hindgut (13) CNS |
CNS dorsal vessel |
L2: MBNbs, VNCTh,T, CB, OPC, IPC L3: VNCTh,T, CB, lamina |
| pstgβ-E6.7 | – | CNS | CNS | CNS | L2 MBNbs, VNCTh,T, CB L3: VNCTh,T, CB, pOPC, IPC |
For each transgenic line, tissues positive for lacZ expression are listed, followed by the corresponding mitotic domain number(s). Expression patterns believed to be spurious (unlike endogenous string) are denoted with italics, and are listed only for the pstgβ and pstgHZ vectors. The same spurious vector expression patterns were also observed with most of the PSE-containing reporter genes, but for simplicity they are not listed again in each row. Note that mitotic domains are often only subsets of the tissues listed. Asterisks (*) denote domains that are subdivided during cycles 15 and 16. Slashes (−) denote no significant expression. Abbreviations are as follows: CNS, central nervous system; PNS, peripheral nervous system; MBNbs, mushroom body neuroblasts; VNC, neuroblasts of the ventral nerve cord; CB, neuroblasts of the central brain; neuroblasts of the inner proliferation center (IPC) and outer proliferation center (OPC) of the optic lobe. Note that VNC neuroblasts are subdivided into those located in the thoracic (Th), abdominal (A) and terminal (T) regions of the VNC. The OPC is subdivided into anterior (aOPC) and posterior (pOPC) regions in the third instar (Fig. 4).