FIG. 2.

Translocation of Tir into HeLa cell membranes sequentially increases with time. HeLa cells were infected with wild-type (wt) EPEC E2348/69 for 1 to 4 h and fractionated by the ultracentrifugation method as described in the text. After samples were resolved by SDS-PAGE (10% gel) and transferred to nitrocellulose membranes, immunoblots were probed with anti-Tir monoclonal antibody. Arrows indicate 78- and 90-kDa forms of Tir.