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. 2023 May 7;15(9):2647. doi: 10.3390/cancers15092647

Figure 4.

Figure 4

Tumor-associated macrophages/microglia express high levels of C3aR and VEGF in GBM. (a) Frozen tissue sections of GBM were sequentially stained for CD18 (mouse antibody (Mab), Green) and C3aR (rabbit Ab Thermo, Red), Alexa 488 or 594-conjugated secondary antibodies and counterstained with DAPI (to stain nuclei in blue). The arrows indicate C3aR/CD18+ cells while the arrowhead points to a mast-cell-like C3aR/CD18-cell. (b,c) Double staining of GBM paraffin wax sections for C3aR (Mab, clone D12 Santa Cruz) and either (b) anti-CD163 (Clone 10D6) or (c) Mab anti-CD68 (clone 514H12, Leica). Arrowheads point to C3aR cells. (d) Frozen tissue sections of GBM were double-stained for C3aR (Mab, clone D12), Green) and VEGF (rabbit Ab Millipore, Red), Alexa 488 or 594-conjugated secondary antibodies and counterstained with DAPI. Arrows point to TAM-like amoeboid cells double-stained for C3aR and VEGF. All images are shown at ×400 magnification.