Figure 6.

Activation of complement system via the alternative pathway (Bb-dependent) in GBM. Paraffin wax sections of GBM were single-stained for several components of the C system using (a) rabbit anti-native C3 (inset is the negative control of irrelevant antibody staining); (b) rabbit anti-C3c (fragment generated after C activation); (c) rabbit anti-anaphylatoxin C3a; (d) rabbit anti-Bb neoepitope (generated after activation of the alternative pathway); (e) Paraffin wax sections of a GBM case were double-stained for C3a (clone K13/16, red) and C3aR (clone D12, brown) and (f) rabbit anti-TGF-β. Immunoperoxidase and hematoxylin stains were applied. The bar indicates 50 μm. All images are shown at ×400 magnification.