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. 2023 May 5;15(9):2626. doi: 10.3390/cancers15092626

Figure 1.

Figure 1

c-Met signaling in cancer cells is associated with neutrophil activity in brain metastasis (A) Brain metastasis-free survival and brain bioluminescence of SKBrM3-Tet-shMet cells with or without doxycycline treatment after tumor inoculation. Doxycycline was given in drinking water immediately after injection. I.C., intracardiac. (B) Representative picture of mice at day 0 and day 28 after tumor inoculation. (C) Flowchart of pathway analysis. (D) A list of 200 top-upregulated genes was inputted into QIAGEN Ingenuity Pathway Analysis software for canonical pathway enrichment analysis. Pathways enriched in 231BrM-Tet-shMet and SKBrM3-Tet-shMet without doxycycline compared to cells treated with doxycycline (1 µg/mL). (E) Representative picture of ELA2 staining in brain metastasis from mice. Scale bar: 20 µm (F) Left, ELA2 staining of brain metastasis sample from breast cancer patients. Scale bar: 100 µm. Right, quantification of neutrophil distribution pattern (** p  <  0.01, or *** p < 0.001).