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. 2023 May 5;15(9):2626. doi: 10.3390/cancers15092626

Figure 2.

Figure 2

c-Met signaling regulates expression of key neutrophil granulopoiesis and homeostasis factors (A) Heatmap of G-CSF (CSF3), GM-CSF (CSF2), and CXCL1/2/5 expression in cells with or without doxycycline treatment from RNA sequencing data. (B) Brain metastasis-free survival analyses of G-CSF and CXCL1 in 710 breast cancer patients using the combined GEO databases (GSE12276, GSE2034, GSE2603, GSE5327, and GSE14020). Patients were divided into two groups based on the expression status of the gene in their primary tumors. (C) Scheme of conditioned medium (CM) preparation. Notably, there is no doxycycline in DOX+ CM. Cells were pretreated with doxycycline for 48 h before CM preparation. (D) ELISA of CXCL1/2, GCSF, and GM-CSF in 231BrM-Tet-shMet and SKBrM3-Tet-shMet CM. (E) ELISA of CXCL1 and GCSF in MCF7, MCF7-cMet, MDA-MB-453 and MDA-MB-453-cMet CM (* p  <  0.05, ** p  <  0.01, or *** p < 0.001).