Table 2.
Sequences of the primers and hydrolysis probes used for each target region of the multiplex qPCR–omIA gene (A. pleuropneumoniae), p102 gene (M. hyopneumoniae), and kmt1 gene (P. multocida), and the respective amplifier sizes.
| Gene | N° of Access on GenBank | Primers and Probes | The Nucleotide Sequence (5′ → 3′) | Amplicon Size (bp) | Reference |
|---|---|---|---|---|---|
| omIA | [gb|NC_009053|] | F_App_ omIA |
AGTGCTTACCGCATGTAGTGGC | 153 | [41] |
| R_App_ omIA |
TTGGTGCGGACATATCAACCTTA | ||||
| P_App_ omIA |
5′-Cy5-CGATGAACCCGATGAGCCGCC-3′-IB®RQ | ||||
| p102 | [gb|AE017332.1|] | F_Mhp_p102 | 5′-TAAGGGTCAAAGTCAAAGTC-3′ | 150 | [42] |
| R_Mhp_p102 | 5′-AAATTAAAAGCTGTTCAAATGC-3′ | ||||
| P_Mhp_p102 | 5′-FAM-AACCAGTTTCCACTTCATCGCC-3′-BHQ1 | ||||
| kmt1 | [gb|NC_017027.1|] | F_Pmt_kmt1 | 5′-GGGCTTGTCGGTAGTCTTT-3′ | 148 | [43] |
| R_Pmt_kmt1 | 5′-CGGCAAATAACAATAAGCTGAGTA-3′ | ||||
| P_Pmt_kmt1 | 5′-TexRd- CGGCGCAACTGATTGGACGTTATT3′-IB®RQ |
omIA: target gene for Actinobacillus pleuropneumoniae; p102: target gene for Mycoplasma hyopneumoniae; kmt1: target gene for Pasteurella multocida type A; F: forward primer; R: reverse primer; P: probe; bp: base pairs.