Figure 3.

Effects of the AIs, Ana, Let, and Exe, as well as of CBD and their combinations on aromatase protein levels. MCF-7aro cells were stimulated with T (1 nM) and treated with the AIs (10 µM), CBD (5 µM), or their combinations, over 3 days. Cells treated only with T were used as control. A representative Western blot of aromatase and β-actin, as well as the densitometric analysis of aromatase expression levels after normalization with β-actin levels, used as loading control, are presented. Statistically significant differences between treated cells and T are expressed as * (p < 0.05) and *** (p < 0.001). The original Western blots are represented in Supplementary Figure S1A.