Figure 8.

Effects of Exe or CBD and their combination on MCF-7aro cell viability (A), apoptosis (B), and mRNA transcript levels of ESR1, TFF1, AREG, and EGR3 (C) in the presence of T (1 nM) or E₂ (1 nM). Cells were stimulated with E₂ or T and treated with the Exe (10 µM), CBD (1 and 5 µM), or their combinations for 2 or 3 days. Cells treated only with T or E₂ were used as control, representing 100% of cell viability, and cells treated with STS were considered as positive control. mRNA transcript levels for ESR1, AREG, EGR3, and TFF1 genes were analyzed in relation to the housekeeping gene β-actin. Statistically significant differences between treated cells and E₂, or between E₂- and T-treated cells are expressed as * (p < 0.05) and ** (p < 0.01), while differences between the combinations and Exe alone are represented as ## (p < 0.01), and the differences between the combinations and CBD as $$ (p < 0.01).