Table 4.
Selected mean (±PSE) fatty acid composition (% of total fatty acids by weight) of feces from Channel Catfish fed diets supplemented with 4% of either soybean oil (SBO), SBO enriched with conjugated linoleic acids (CLA-SBO), catfish offal oil (COO), flaxseed oil (FXO), menhaden fish oil (MFO), or poultry fat (PF) 1.
| Diets | ||||||||
|---|---|---|---|---|---|---|---|---|
| Fatty Acid | SBO | CLA-SBO | COO | FXO | MFO | PF | PSE 2 | Pr > F |
| 14:0 | 0.7 bc | 0.6 c | 1.0 b | 0.9 bc | 3.2 a | 0.9 bc | 0.1 | <0.001 |
| 16:0 | 20.0 d | 20.5 cd | 22.7 bc | 22.3 cd | 25.0 b | 30.0 a | 0.9 | <0.001 |
| 18:0 | 5.7 | 6.2 | 6.4 | 6.9 | 6.2 | 7.6 | 0.6 | 0.388 |
| 20:0 | 0.4 | 0.5 | 0.4 | 0.3 | 0.4 | 0.3 | 0.06 | 0.213 |
| 22:0 | 0.6 a | 0.6 a | 0.4 b | 0.5 b | 0.5 b | 0.3 c | 0.04 | 0.001 |
| ∑ SFA 3 | 27.4 b | 28.5 b | 30.9 b | 30.9 b | 35.3 a | 39.1 a | 1.4 | <0.001 |
| 16:1n-7 | 0.8 d | 1.0 cd | 1.4 c | 0.6 d | 3.2 a | 2.1 b | 0.2 | <0.001 |
| 18:1n-9 | 18.2 b | 18.6 b | 24.4 a | 18.8 b | 15.6 c | 20.3 b | 0.8 | <0.001 |
| 18:1n-7 | 2.1 b | 2.1 b | 2.3 b | 2.2 b | 2.8 a | 2.1 b | 0.1 | 0.019 |
| 20:1n-9 | 0.5 b | 0.5 b | 0.8 a | 0.5 b | 0.5 b | 0.4 b | 0.04 | <0.001 |
| 22:1n-11 | ND | 0.1 | ND | ND | 0.1 | 0.1 | 0.1 | 0.690 |
| ∑ MUFA 4 | 21.5 c | 22.5 c | 29.0 a | 22.1 bc | 22.3 bc | 25.2 b | 1.03 | 0.002 |
| CLA-9c,11t | ND | 0.5 | ND | ND | ND | ND | 0.03 | ----- |
| CLA-10t,12c | ND | 0.3 | ND | ND | ND | ND | 0.03 | ----- |
| ∑CLA isomers 5 | ND | 0.8 | ND | ND | ND | ND | 0.05 | ----- |
| 18:2n-6 | 45.6 a | 43.6 a | 35.6 bc | 37.0 b | 34.1 bc | 32.0 c | 1.2 | <0.001 |
| 18:3n-3 | 4.7 b | 4.3 bc | 3.4 d | 9.9 a | 3.8 cd | 3.1 d | 0.3 | <0.001 |
| 20:2n-6 | 0.2 | 0.1 | 0.4 | 0.1 | 0.1 | 0.1 | 0.1 | 0.056 |
| 20:4n-6 | 0.2 | 0.2 | 0.3 | ND | 0.1 | 0.2 | 0.1 | 0.876 |
| 20:5n-3 | ND | 0.1 b | ND | ND | 1.8 a | ND | 0.1 | <0.001 |
| 22:5n-3 | ND | ND | ND | ND | ND | ND | ----- | ----- |
| 22:6n-3 | ND | 0.2 b | 0.3 b | ND | 2.4 a | ND | 0.2 | <0.001 |
| ∑ n-3 6 | 4.7 c | 4.7 c | 3.7 cd | 9.9 a | 8.0 b | 3.1 d | 0.3 | <0.001 |
| ∑ n-6 7 | 46.0 a | 43.9 a | 36.3 bc | 37.1 b | 34.3 bc | 32.7 c | 1.2 | <0.001 |
| ∑ n-3 LC-PUFA 8,9 | ND | 0.3 b | 0.3 b | ND | 4.2 a | ND | 0.3 | <0.001 |
| ∑ n-6 LC-PUFA 8,10 | 0.4 | 0.2 | 0.9 | 0.1 | 0.2 | 0.3 | 0.2 | 0.081 |
| n-3/n-6 | 0.1 c | 0.1 c | 0.1 c | 0.3 a | 0.2 b | 0.1 c | 0.006 | <0.001 |
1 Values are means of three replicates per treatment analyzed in duplicate; ND denotes that the fatty acid was not detected; a dash denotes that the indicated statistic could not be computed. Means within rows with different letters are significantly different (p ≤ 0.05). Fisher’s least significance difference test was used to identify specific differences. 2 Pooled standard error (PSE). 3 Total saturates (SFA) included 14:0, 16:0, 18:0, and 20:0. 4 Total monounsaturates (MUFA) included 16:1, 18:1, 20:1, and 22:1. 5 Total CLA isomers included 9c, 11t; and 10t, 12c. 6 Total n-3 fatty acids included 18:3n-3, 20:5n-3, 22:5n-3, and 22:6n-3. 7 Total n-6 fatty acids included 18:2n-6 (excluding CLA isomers), 20:2n-6 and 20:4n-6. 8 Long-chain polyunsaturated fatty acids (LC-PUFA). Fatty acids with 20 or more carbons and four or more double bonds. 9 Total n-3 LC-PUFA included 20:5n-3, 22:5n-3, and 22:6n-3. 10 Total n-6 LC-PUFA included 20:2n-6 and 20:4n-6.