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. 2023 Apr 22;15(9):2415. doi: 10.3390/cancers15092415

Figure 4.

Figure 4

Gas6/Axl induces MAPK signaling and PRAME expression. (A) Levels of phosho−ERK1/2 (pERK1/2) were assessed by Western blotting upon stimulation of HepG2, Hep3B, HLF, HLE and SNU475 cells with 500 ng/mL Gas6 for 15 min. (B) Quantification of pERK1/2 levels by densitometry (na: not applicable). The abundance of pERK1/2 in the absence of Gas6 was set to a value of 1 for each liver cancer cell line. (C) PRAME expression together with pERK1/2 and total ERK1/2 levels in HLF cells were assessed by Western blotting upon administration of 1 µM of either MEK inhibitors Trametinib or U0126. The expression of actin is shown as loading control (A,C). (D) pERK1/2 and PRAME expression were quantified by densitometry. The expression of DMSO−treated control cells was set to a value of 1. Data are expressed as mean ± SD. ns: p > 0.05; *: p ≤ 0.05; **: p ≤ 0.01; ***: p ≤ 0.001.