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. 2023 May 7;12(9):1337. doi: 10.3390/cells12091337

Figure 1.

Figure 1

CircHTT(2-6) is the predominant circRNA expressed from the HTT gene. (A) Linear vs. circular RNA splicing, with exons shown as coloured arrows. (B) All known circRNAs from the HTT gene from circBase and CIRCpedia v2. HTT gene locus shown at bottom, with vertical blue lines representing exons. Blue lines represent individual circRNAs that arise from the HTT gene, with circHTT(2-6) highlighted in red. CAG repeat region in exon 1 is highlighted. (C). Agarose gel electrophoresis showing PCR amplicons for circHTT(2-6) and HTT mRNA from SH-SY5Y cells with and without RNase R treatment prior to cDNA synthesis. NTC: non-template control. (D) Sanger sequencing chromatogram of circHTT(2-6) amplicon showing backsplice junction between exons 6 and 2.