Figure 4.

RP-832c treatment blocks the “cytokine storm” in an established late model of BLM-induced lung fibrosis. (A) A schematic of the animal study in which mice were challenged with 2.5 U/kg body weight dose of BLM on day 0. A total of 14 days post-BLM challenge, mice were treated with either a vehicle or 10 mg/kg RP-832c (QD) for an additional 21 days. (B) The upper panel shows 2× representative images of Masson’s Trichrome staining of whole lung tissue, and the lower panel contains representative 20× images of Masson’s Trichrome-stained lung tissue. (C) A bar graph for the modified Ashcroft scoring of the lung tissues. (D) Representative 20× and 40× images of immunofluorescence staining of lung tissue sections with anti-F4/80 (red) and Anti-CD206 (green). (E) Quantification of F4/80 & CD206 double-positive macrophages in the IF staining of the lung tissues. Quantification was performed by measuring counts in 10 separate fields using Metamorph imaging software. (F) RT-PCR of IL-8 functional homologs (CXCL-1, CXCL-2), inflammatory cytokine markers (CD206, CD86, IL-6, IL-10, IFN-γ, TNF-α, IL-1B, iNOS), and fibrosis markers (TGF-β, and MMP-13) using RNA extracted from FFPE tissue sections. S. E. **** p < 0.0001, *** p < 0.001, ** p < 0.01 and * p < 0.05 were considered significant.