Table 2.

Detailed clamping PCR results for 28 ESR1 mutations.

Case	AA Change	SNV	VAF (%)	Mutant Copy Number (per Well)	Total Copy Number (per Well)
1	Y537S	1610A>C	0.0025	2.8	111,150
38	Y537S	1610A>C	0.0031	3.6	116,800
49	Y537S	1610A>C	0.0022	2.8	129,000
59	Y537S	1610A>C	0.0028	3	108,800
84	Y537S	1610A>C	0.0018	2.8	158,000
117	Y537S	1610A>C	0.0023	2.4	106,540
119	Y537S	1610A>C	0.0021	2.6	121,200
120	Y537S	1610A>C	0.0024	2.6	108,500
121	Y537S	1610A>C	0.0037	3.8	102,200
139	Y537S	1610A>C	0.1580	6.8	4304
165	Y537S	1610A>C	0.0053	2.6	48,640
173	Y537S	1610A>C	0.0106	3	28,320
177	Y537S	1610A>C	0.0025	2.8	112,800
194	Y537S	1610A>C	0.0026	2.6	101,700
202	Y537S	1610A>C	0.0028	2.6	91,280
132	Y537S	1610A>C	0.0034	2.8	81,280
132	D538G	1613A>G	0.0034	2.8	81,280
2	D538G	1613A>G	0.0041	2.6	63,840
7	D538G	1613A>G	0.0119	2.8	23,520
68	D538G	1613A>G	0.0110	4.8	43,520
144	D538G	1613A>G	0.0422	2.8	6640
145	D538G	1613A>G	0.0060	2.8	46,690
146	D538G	1613A>G	0.0111	3	27,040
158	D538G	1613A>G	0.0053	3	56,480
187	D538G	1613A>G	0.0053	2.8	52,800
207	D538G	1613A>G	0.0111	2.6	23,520
211	D538G	1613A>G	0.0039	3.8	96,480
212	D538G	1613A>G	1.6260	36	2178

AA—amino acid, SNV—single nucleotide variant, and VAF—variant allele frequency.