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RT-PCR analysis of cytokine mRNA obtained from antigen-stimulated popliteal lymph node cells from mice infected with 106 (A) or 102 (B) L. major metacyclic promastigotes. Results are expressed as the fold increase in IFN-γ (black bars), IL-12 (hatched bars), and IL-4 (gray bars) mRNA in antigen-stimulated cultures compared to that in unstimulated controls.
