Table 5.

Effect of dietary selenium source and Eimeria infection on the tight junction and mucin gene expression of broiler chickens ¹.

Item	Diet 2			SEM	Infection 3		SEM	p-Value
	CON	SS	SY		Un-Infected	Infected		Diet	Infection	D × I
Duodenum
JAM-2	0.072 ab	0.013 a	0.15 b	0.036	0.034	0.123	0.029	0.037	0.058	0.084
OCLN	0.164	0.097	0.187	0.046	0.114	0.185	0.038	0.367	0.197	0.398
ZO-1	0.207	0.099	0.341	0.073	0.151	0.281	0.062	0.085	0.136	0.136
MUC-2	1.396	0.846	1.392	0.374	1.04	1.382	0.322	0.526	0.446	0.256
Jejunum
JAM-2	0.048	0.048	0.048	0.017	0.047	0.046	0.014	0.835	0.936	0.083
OCLN	0.078 a	0.078 a	0.129 b	0.013	0.111	0.079	0.01	0.008	0.033	0.137
ZO-1	0.146	0.116	0.170	0.031	0.138	0.151	0.026	0.464	0.718	0.795
MUC-2	4.465	4.270	4.955	0.979	6.205	2.922	0.799	0.879	0.008	0.960

¹ Transcript levels of junctional adhesion molecule 2 (JAM2), occludin (OCLN), zonula occludens 1 (ZO1), and mucin 2 (MUC-2) in duodenal and jejunal mucosa were measured by quantitative RT-PCR and gene expression was analyzed using the 2^−∆∆Ct method. SEM; pooled standard error of the mean. ^a,b Values in a row with different superscripts differ significantly (p < 0.05). ² Values are the mean of 12 replicates per treatment: CON: Se-un-supplemented diet, SS: inorganic Se-supplemented diet (0.3 mg/kg as sodium selenite), and SY: organic selenized yeast-supplemented diet (0.3 mg/kg, Sel-Plex^®, Alltech). ³ Values are the mean of 18 replicates per treatment; chickens were infected by oocysts of mixed Eimeria (E. tenella, E. acervulina, and E. maxima) using oral gavage on day 16 with 15,000 oocysts/chicken.