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. 2023 Apr 24;15(9):2437. doi: 10.3390/cancers15092437

Figure 2.

Figure 2

Overexpression of miRNA-23c and miRNA-4328 results in reduced prostate cancer cell growth in culture. (a,b) Relative levels of miRNA-23c and miRNA-4328 (mean ± standard deviation, n = 2–3) determined by reverse transcription and quantitative polymerase chain analysis after stable overexpression in the 22Rv1 and PC-3 cell lines. WT, wildtype; NTC, non-target control. (c) Relative proliferation rate of 22Rv1 cells and PC-3 cells overexpressing miRNA-23c, miRNA-4328 and NTC sequence, determined by measuring the fluorescent intensity of turbo green fluorescent protein (Spectramax i3x reader) at 24, 48, 72 and 96 h after seeding in relation to the intensity at 15 and 18 h, respectively (n = 12). (d) Relative number of 22Rv1 and PC-3 cells at 72 h after seeding (CellTiter-Glo® Luminescent Cell Viability Assay). The experiment was repeated twice with six replicates each per cell line. ** p < 0.01, *** p < 0.001, **** p < 0.0001 (unpaired t-test).