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. 2023 Apr 24;15(9):2437. doi: 10.3390/cancers15092437

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of miRNA-23c overexpression on PC-3 tumor growth and histology in vivo. (a) Tumor size after subcutaneous injection of miRNA-23c overexpressing PC-3 cells and non-target control cells (NTC) in nude mice. (b) Tumor weight at endpoint (day 37). (c) Tumor histology at endpoint, as demonstrated by representative tissue sections from miRNA-23c overexpressing and NTC PC-3 tumors stained by hematoxylin and eosin (H&E) or CD31, as indicated. (d) Relative expression level of miRNA-23c in tumors at endpoint and (e) vessel density (%) in NTC and miRNA-23c overexpressing tumors at endpoint, according to volume density of CD31 stained structures. Data are shown as mean ± SD. **** p < 0.0001, ns p > 0.05 (unpaired t-test and Mann–Whitney U-test).