Figure 2.

Holographically designed lens, high intensity focused ultrasound transducer, and absorbing gel spheroid holder used in hyperthermia experiments. (A) Schematic of the spheroid exposure system with its dimensions. (B) 3D-printed lens with the holder by which it is mounted on top of the transducer. (C) The mold was used to form the absorbing gel phantom with the three thermocouples located 0.5 mm above the protrusion used to form each well. (D) The mold was filled with IEC tissue-mimicking gel, with 3 miniature wells into which a spheroid could be placed (inset). A gel lid closed the phantom (E) Experimental setup to expose the spheroids to ultrasound hyperthermia.