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. 2023 Apr 23;15(9):2427. doi: 10.3390/cancers15092427

Figure 8.

Figure 8

High-dosage NMN promoted ferroptosis of lung adenocarcinoma cells through NAM-overload-mediated SIRT1–AMPK–ACC pathway. (A) Mitochondrial iron concentration was determined via Mito-FerroGreen labeling using cell immunofluorescence in indicated groups; scale bar: 200 μm. (B) ROS production in A549 and SPCA1 cells detected via DCFH-DA, measured using immunofluorescence analysis in indicated groups; scale bar: 200 μm. (C) Lipid peroxidation was detected in A549 and SPCA1 cells measured using BODIPY 581/591 C11 stain (BODIPY-C11); scale bar: 200 μm. (D,E) PUFA levels in A549 (D) and SPCA1 (E) cells were examined using ELISA assay in indicated groups (n = 5). ** p value < 0.01; **** p < 0.0001.