Fig. 5 |. High site occupancy and remodeler autonomy quantifies pioneering criteria.

a, Schematic of a TF trajectory between two specific chromatin targets showing the search time ${\tau }_{\text{search}}$, stable ${\tau }_{\text{sb}}$ and transient ${\tau }_{\text{tb}}$ dwell times, and ${\tau }_{\text{free}}$. The TF molecule dissociates from a specific target, and samples nonspecific sites for a number of trials before encountering the next specific target. The ${\tau }_{\text{search}}$ equation is indicated (Methods). b, Key SPT and $N_{\text{monomers}}$ parameters measured in this study and $N_{\text{sites}}$ from the literature^17,54 are used to calculate occupancy levels for GAF and HSF. c, Violin plots of GAF ChIP-seq peak intensities (analysis of table s3 from Fuda et al.)¹⁷ in hemocyte-like S2 cells plotted by the number of nonoverlapping GAGAG elements between the start and end coordinates of each peak identified with HOMER¹⁰⁸.