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. 2023 May 11;12(1):2208682. doi: 10.1080/22221751.2023.2208682

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© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 3. — Detection of MPXV IgG and neutralizing antibodies by ELISA and PRNT. (A) Plaque reduction assay of serum against MPXV. (B) The inhibitory curve for positive sera. (C) Neutralizing activity of sera from individuals (N = 30) who were born before 1981 and after 1981. (D) Geometric mean titre (GMT) of neutralizing antibodies against MPXV. (E) MPXV IgG antibodies of sera from individuals (N = 30) who were born before 1981 and after 1981 by ELISA. (F) GMT of IgG antibodies by ELISA. There were two people born in the same year such as 1968, 1969, 1977, 1992, 1996, 1997, and 1999 respectively, which resulted in 23 data points shown (7 data points less) in Figure 3C and E. Data shown are geometric mean titres. Error bars represent geometric standard deviations. Data were analysed by the one-way ANOVA tests and t-test analysis. P < 0.05 was considered statistically significant. *P < 0.05.