Figure 3.

In vitro and in vivo validation of mTOR inhibitor TKA001. (A) Western blot of HT1080 cells pre-treated with increasing concentrations of TKA001. p-S6 indicates phospho-S6 (S240/244), and p-AKT indicates phospho-AKT (S473). The blots are shown as short and long exposure times, total AKT, and vinculin loading control. (B) Proliferation assays of TKA001 treatment on HT-1080 cells. Half maximal inhibitory concentration (IC₅₀) = 200 nM. (C) Proliferation assays of TKA001 treatment on HeLa cells. IC₅₀ = 1 µM. (D,E) Feeding 10, 100, or 200 µM TKA001 increased the lifespan of C. elegans. Two independent biological trials. Control = 0.2% DMSO. p-value determined with Log Rank. Raw data, statistics, and additional trials are in Supplementary Table S2.