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Western blot of mutanolysin surface preparations from 37- and 46°C-grown E. faecalis isolates probed with anti-Ace A polyclonal immune rabbit serum. Lanes: 1 and 2, protein extracts from 37- and 46°C-grown MC02152; 3 and 4, protein extracts from 37- and 46°C-grown END6; 5 and 6, protein extracts from 37- and 46°C-grown V583; 7 and 8, protein extracts from 37- and 46°C-grown SE47b; and 9, molecular mass standards.
