Figure 2.

Analysis of Nrf2 nuclear translocation. Western blot and densitometric analysis of Nrf2 protein levels in nuclear extracts of U373 and U373-Tat cells after 4 h of treatment with 100 μg/mL of Nat-bLf or Holo-bLf. Data are calculated relative to the internal housekeeping gene (Lamin A) and are expressed as the means ± SEM. One-way ANOVA, followed by Tukey’s test, was used to determine significant differences. * p ≤ 0.05 vs. CTRL; °° p ≤ 0.01 and °°° p ≤ 0.001 vs. Tat; ^ p ≤ 0.05 between Tat and CTRL.