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. 2023 Apr 27;24(9):7947. doi: 10.3390/ijms24097947

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Cell viability measurement by MTT assay on co-cultures of SH-SY5Y and U373 or U373-Tat cells, pre-treated with 2 mM N-acetyl cysteine (NAC) (A,B) or 300 μM sulfasalazine (C,D), untreated or treated with 100 μg/mL Nat- or Holo-bLf for 24 h. The histograms show the percentage of living cells, and the rate of reduction was calculated by setting the control (CTRL) equal to 100%. One-way ANOVA, followed by Tukey’s test, was used to determine significant differences. *** p ≤ 0.001 and **** p ≤ 0.0001 vs. CTRL; ° p ≤ 0.05 and °°° p ≤ 0.001 vs. Tat; ^^^ p ≤ 0.001 and ^^^^ p ≤ 0.0001 between Tat and CTRL; + p ≤ 0.05, ++ p ≤ 0.01, +++ p ≤ 0.001 and ++++ p ≤ 0.0001 between treatment with SSZ or NAC and without SSZ or NAC.