TABLE 3.
Inhibition of adherence of 46°C-grown E. faecalis clinical isolates V583 and MC02152 to ECM proteins by IgGs (20 μg/ml) purified from anti-Ace A (OG1RF-derived) rabbit immune serum
| ECM | % of cells bounda
|
|||||
|---|---|---|---|---|---|---|
| V583
|
MC02152
|
|||||
| No IgG | PI | Anti-Ace | No IgG | PI | Anti-Ace | |
| CI | 29.2 ± 1.87 | 28.1 ± 2.84 | 3.2 ± 0.17 | 26.0 ± 1.61 | 24.4 ± 1.31 | 2.9 ± 0.46 |
| CIV | 38.1 ± 8.66 | 33.6 ± 6.81 | 2.5 ± 0.17 | 27.7 ± 0.39 | 26.5 ± 1.84 | 2.3 ± 0.13 |
| LN | 29.4 ± 1.96 | 27.6 ± 3.53 | 2.7 ± 0.78 | 26.7 ± 1.22 | 23.5 ± 0.76 | 1.9 ± 0.52 |
| BSA | 0.7 ± 0.10 | 0.6 ± 0.07 | 0.6 ± 0.01 | 0.6 ± 0.12 | 0.5 ± 0.19 | 0.6 ± 0.17 |
a
Values are mean percentages of cells bound ± standard deviations for six wells. Results are representative of three independent experiments. PI, IgGs purified from preimmune rabbit serum; Anti-Ace, IgGs purified from polyclonal anti-Ace A rabbit immune serum.