Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Apr 24;24(9):7782. doi: 10.3390/ijms24097782

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

(a) Immunofluorescence staining of PDGFRβ in U2OS cells that were treated with control siRNA (“siCtr”) or depleted of TRIM21 protein (“siTRIM21”). Single channel staining of PDGFRβ is presented (red) and merged with nuclear staining with DAPI (blue). (b) Immunofluorescence staining of endogenous TRIM21 (green) shows weak cytoplasmic distribution in control cells (“siCtr”) and efficient knockdown in (siTRIM21) cells; staining merged with nuclei (blue) images are also presented. Confocal images in (a,b) were taken with 63× oil objective and zoom 0.5. (c,d) Immunofluorescence staining of PDGFRβ in myc-TRIM21-inducible U2OS cells cultured in 10% FBS after induction of TRIM21 before (c) or after (d) stimulation with PDGF-BB for 5 min. PDGFRβ staining is in the red; TRIM21 (myc) staining is in green; chromatin counterstained with DAPI is in the blue. Merged images for all three channels are presented on the right. Confocal images in (c,d) were taken with 63× oil objective and zoom 0.7. Scale bars are 10 µm.