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. 2023 Apr 25;24(9):7825. doi: 10.3390/ijms24097825

Figure 3.

Figure 3

MiR-155-5p targets the 3′UTR of Jade-1. (A) The consensus binding site for miR-155-5p at positions 1257–1264 of the Jade-1 3′UTR. (B) Luciferase activity with the WT Jade-1 3′UTR and a mutant site compared to scramble negative control. (C) Protein expression of Jade-1 significantly increased following knockdown of miR-155-5p in 786-O and Caki-1 cell lines. Representative Simple Western™ images inset to the right: the first lane of each pair corresponds to JADE-1 and the second is total protein detected for the same lane. * p < 0.05, ** p < 0.01.