TABLE 3.

β-Gal activity of the gonococcal norB′-′lacZ fusion

Strain	Relevant genotype	β-Gal activity (Miller units ± SEM)a
		Aerobic cultures		Anaerobic cultures
		+NO2	−NO2	+NO2	−NO2
RUG7021	Promoterless lacZ	0.1 ± 0.01	0.1 ± 0.01	0.4 ± 0.11	0.1 ± 0.03
RUG7500	norB′-′lacZ	23 ± 1.5	20 ± 1.6	1,798 ± 18.4	34 ± 1.9
RUG7501	norB′-′lacZ aniA::ermC	20 ± 1.2	21 ± 0.5	42 ± 6.4	29 ± 0.5
RUG7502	norB′-′lacZ tacP::aniA	164 ± 11	19 ± 0.1	1,094 ± 33	41 ± 9.5
RUG7503	norB′-′lacZ narP::ermC	21 ± 0.5	21 ± 0.4	1,081 ± 36	22 ± 2

^aAerobic cultures were grown in GCK broth with shaking in baffled flasks in the presence (+) or absence (−) of 2 mM sodium nitrite (NO₂). Anaerobic cultures were grown on plates with (+) or without (−) disks saturated with 20% (wt/vol) sodium nitrite (NO₂).