Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The Matsutani et al. (28) method for isolating repeated DNA. Genomic DNA was subjected to hydrodynamic shearing, alkali denaturation, neutralization, and a brief period of reannealing followed by quick cooling on ice to favor intra- and intermolecular hybridization of repetitive DNA. Single-stranded DNA was digested with S1 nuclease, and remaining double-stranded DNA was cloned into pUC18. Arrows in bold indicate repetitive DNA.
