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. 2023 Apr 25;24(9):7830. doi: 10.3390/ijms24097830

Figure 7.

Figure 7

Effect of treadmill exercise on FNDC5/integrinαVβ5-mediated BDNF levels in MPTP-induced chronic PD model and verification of FNDC5–integrin αV β5 interactions. (A) Protein levels of FNDC5, integrin αV, integrin β5 and BDNF were examined with Western blotting. (B–E) Quantification of FNDC5 (F(2,6) = 13.59, p = 0.006), integrin αV (F(2,6) = 58.63, p < 0.001), integrin β5 (F(2,6) = 27.36, p = 0.001), and BDNF (F(2,6) = 33.06, p < 0.001) levels (n = 3). (F,G) Representative immunofluorescence images and summarized data of staining for integrin (t(10) = 33.18) in HT22 (hippocampal neuron cell line) cells. Scale bar = 100 μm, n = 6. (H,I) Interactions between FNDC5 and integrin proteins (F(2,6) = 45.77, p < 0.001) were examined by Co-IP, n = 3. Data represent the mean ± SEM; * p < 0.05, ** p < 0.01 *** p < 0.001 vs. control, # p < 0.05 vs. MPTP group. M (MPTP), ME (MPTP + exercise), IP (immunoprecipitation), and IB (immunoblotting).