TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype, relevant phenotype, or selection marker | Source or reference |
|---|---|---|
| Strains | ||
| E. coli XL1-Blue MRF′ | Δ(mcrA) 183 Δ(mcrCB-hsdSMR-mrr) 173 endA1 sup E44 thi-1 recA1 gyrA96 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tetr)] | Stratagene |
| M. catarrhalis | Wild-type serotype A | ATCC 25238 |
| M. catarrhalis 2951 | Wild-type clinical isolate, serotype A | This study |
| M. catarrhalis 2951 galE | UDP-glucose 4-epimerase deficient | This study |
| Plasmids | ||
| pMCSau2 | Ampicillin | This study |
| pMCApoA | Ampicillin | This study |
| pMCPvu1a | Ampicillin | This study |
| pMCgalE | Ampicillin | This study |
| pMCgalEBBR | Ampicillin, spectinomycin | This study |
| pUC18 | Ampicillin | Pharmacia Biotech |
| pUC19 | Ampicillin | Pharmacia Biotech |
| pTAV1 | Ampicillin | 6 |
| pMC2951galETA | Ampicillin | This study |
| pABR3 | Ampicillin, spectinomycin | 2 |
| pD2galE | Kanamycin | 31 |