FIG. 1.

E. coli transformed with hge-d (left panel) or ee-o (right panel) expresses a 68- or 145-kDa antigen, respectively, as determined by protein immunoblotting. The blots were reacted with the following primary antibodies: human anti-HGE agent (lanes 1), horse anti-HGE agent BDS strain (lanes 2), human anti-E. chaffeensis (lanes 3), dog anti-E. canis (lanes 4), human anti-Borrelia burgdorferi (lanes 5), and human anti-Babesia microti (lanes 6). Lanes 7 and 8 were reacted with normal human and horse sera, respectively. The location of the 68-kDa protein is indicated on the left.