FIG. 5.

Immunoelectron microscopic visualization of AnkA with monoclonal antibody IE3 in HL-60 cells infected with HGE agent (Webster strain). Bars, 0.5 μm. (A) The label is localized in the cytoplasm of the ehrlichiae, both reticulate (r) and dense-cored (d) cells, and on condensed chromatin of the host cell nucleus (arrowheads). (B) In reticulate cells, the cytoplasm is labeled and many gold particles are aligned along the DNA fibrils of the nucleoid (arrowheads). (C) In an apoptotic cell, condensed chromatin of the apoptotic nucleus is heavily labeled.