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. 2023 May 10;39(4):290–299. doi: 10.1089/jop.2022.0139

FIG. 3.

FIG. 3.

HPLC chromatograms of cytosolic fractions (from 100 mm dishes) and culture media (from 12-well plates). ARPE-19 cells were treated with 125I-Ang-(1–12) for 4 h at 37°C. The cells and culture media were collected after 4 h of exposure. The radiolabeled substrate and its metabolic products were analyzed by HPLC. Chromatograms; (A) pooled cytosolic fractions, (B) culture medium in the presence of mAb, and (C) culture medium in the absence of mAb. Details are in Methods section.